普諾賽KYSE-150 (人食管鱗癌細胞)
- 品名: 普諾賽KYSE-150 (人食管鱗癌細胞)
- 型號: CL-0638
- 產品詳情
- 規格參數
產品信息
名稱 | KYSE-150 (人食管鱗癌細胞) (STR鑒定正確) |
別稱 | KYSE 150; KYSE150; Kyse150; KY150 |
背景描述 | Established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice. |
種屬 | 人 |
年齡(性別) | 女;49歲 |
組織來源 | 食管 |
生長特性 | 貼壁細胞 |
細胞形態 | 上皮細胞樣 |
細胞類型 | 腫瘤細胞 |
腫瘤類型 | 食管癌細胞 |
生物安全等級 | 1 |
生長培養基 | RPMI-1640+Ham's F-12+10% FBS+1%P/S |
推薦傳代比例 | 1:4-1:8 |
推薦換液頻率 | 2~3次/周 |
倍增時間 | 13.7 hours (PubMed=1728357); 23 hours (PubMed=25984343); <25 hours, less when serum amount is increased (DSMZ) |
凍存條件 | 凍存液:55% 基礎培養基+40%FBS+5%DMSO溫度:液氮 |
培養條件 | 氣相:空氣,95%;CO2,5%溫度:37℃ |
致瘤性 | Yes, in nude mice |
保藏機構 | DSMZ; ACC-375 |
STR鑒定
STR位點信息
Amelogenin X CSF1PO 12,13 D2S1338 25 D3S1358 15,16 D5S818 12,13 D7S820 10,11 D8S1179 10,15 D13S317 8,11 D16S539 9,11 D18S51 14 D19S433 15,15.2 D21S11 30,31 FGA 21,24 PentaD 10 PentaE 12,18 TH01 7,9 TPOX 8 vWA 16,17 D6S1043 18,20 D12S391 19,22 D2S441 10,11
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