普諾賽M-07e (人巨細胞白血病細胞)
- 品名: 普諾賽M-07e (人巨細胞白血病細胞)
- 型號: CL-0686
- 產品詳情
- 規格參數
產品信息
名稱 | M-07e (人巨細胞白血病細胞) (STR鑒定正確) |
別稱 | M-07E; M-O7e; M07-e; M07e; Mo7e; MO7e; M07E; MO7E |
背景描述 | Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available |
種屬 | 人類 |
年齡(性別) | 女性,6個月 |
組織來源 | 外周血 |
生長特性 | 懸浮細胞 |
細胞形態 | 淋巴母細胞樣 |
細胞類型 | 腫瘤細胞 |
腫瘤類型 | 白血病細胞 |
生物安全等級 | 1 |
生長培養基 | RPMI-1640+10% FBS+8ng/mL GM-CSF+1% P/S |
推薦傳代比例 | 5×10^5-1×10^6cells/mL |
推薦換液頻率 | 2~3次/周 |
倍增時間 | ~40小時 |
凍存條件 | 凍存液:55% 基礎培養基+40%FBS+5%DMSO溫度:液氮 |
培養條件 | 氣相:空氣,95%;CO2,5%溫度:37℃ |
抗原表達情況 | CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR - |
注意事項 | 該細胞為懸浮細胞,請注意離心收集細胞懸液;請勿直接倒掉細胞培養液。 |
保藏機構 | DSMZ; ACC-104 |
STR鑒定
STR位點信息
Amelogenin X CSF1PO 9,10 D2S1338 19,25 D3S1358 16,19 D5S818 11 D7S820 11 D8S1179 13,16 D13S317 10,11 D16S539 11 D18S51 15,20 D19S433 14,15 D21S11 30,32.2 FGA 18,22 PentaD 9 PentaE 12,14 TH01 6,8 TPOX 8 vWA 16,18 D6S1043 13,19 D12S391 23,24 D2S441 14
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